Senior Research Associate, Immunology

<h1>About NewLimit</h1> <p>NewLimit is a biotechnology company working to radically extend human healthspan. We’re developing medicines to treat age-related diseases by reprogramming the epigenome, a new therapeutic mechanism to restore regenerative potential in aged and diseased cells. We leverage functional genomics, pooled perturbation screening, and machine learning models to unravel the biology of epigenetic aging and disease using experiments of unprecedented scale.</p> <h1>Position</h1> <p>NewLimit is seeking a Senior Research Associate to join our Immunology team. One of our initial therapeutic programs is developing an epigenetic reprogramming intervention to rejuvenate aged T lymphocytes for infectious and inflammatory disease applications. In this role, you will help develop culture systems and perform functional assays to evaluate the effectiveness of our therapeutic candidates and enable the development of new medicines.</p> <h1>What you'll do</h1> <ul> <li>Collaborate with our Single Cell Technology, Epigenetic Editing, and Predictive Modeling teams to screen epigenetic reprogramming interventions for aged T cells</li> <li>Support <em>in vivo</em> studies by assisting with tissue processing (e.g., lymphoid and non-lymphoid organs) for downstream cellular and molecular analyses</li> <li>Contribute to the development and execution of immune-mediated disease models, with opportunities for hands-on <em>in vivo</em> work for candidates with relevant experience or interest</li> <li>Isolate human and mouse T cell populations using FACS, MACS, and density gradient separation</li> <li>Develop in vitro assays to assess the therapeutic activity of Treg and conventional T cells</li> <li>Perform flow cytometry to characterize immune cell populations</li> <li>Deliver epigenetic reprogramming perturbations to primary T cells using multiple transgenic manipulation methods (e.g., viral vector, lipofection)</li> </ul> <h1>Requirements</h1> <ul> <li>BS in cell biology, immunology, or a related natural science <strong><em>and</em></strong> 2+ years of work experience in an industry setting</li> <li>Proficiency with mammalian T cell culture</li> <li>Proficiency performing flow cytometry and/or FACS independently, with experience in accompanying data analysis</li> <li>Experience performing T cell functional assays (e.g., proliferation, cytokine, cytotoxicity)</li> <li>Willingness to support <em>in vivo</em> studies through tissue processing, sample preparation, and downstream analyses</li> <li>Exceptional organizational skills, as demonstrated by detailed record-keeping in lab notebooks and an ability to effectively carry out multiple ongoing projects or experiments</li> </ul> <h1>Nice to have</h1> <ul> <li>Hands-on experience with <em>in vivo</em> animal studies, including dosing techniques, dissection, and organ collection</li> <li>Experience with animal models of inflammation and autoimmune disease</li> <li>Experience with primary immune cell isolation using MACS and/or density gradient separation (e.g., Ficoll, Percoll, Lymphoprep, etc.)</li> <li>Experience with high-throughput screening of T cells or other primary immune cells</li> <li>Experience with Treg assays and/or T cell differentiation protocols (e.g., inducible Tregs, Th1/Th2/Th17 polarization)</li> <li>Experience using computational tools to automate routine data analysis (e.g., Python programming, Unix, R)</li> </ul> <h1>Benefits</h1> <p>NewLimit offers generous benefits to ensure our team can focus on our mission:</p> <ul> <li>Health, dental, and vision insurance</li> <li>401(k) with company match</li> <li>Paid parental leave</li> <li>Industry-leading paid time off (PTO) — 20 days/year for all employees + holidays</li> <li>Meals and snacks on-site</li> </ul> <p> </p> <p>Salary Range: $90,000 to $110,000. Compensation for the role will depend on a number of factors, including a candidate’s qualifications, skills, competencies, and experience. NewLimit offers a competitive benefits package, which includes a 401k, healthcare coverage, and 20 days of PTO. Learn more at: <a href="https://www.newlimit.com/careers">https://www.newlimit.com/careers</a>   *This compensation and benefits information is based on Company's good faith estimate as of the date of publication and may be modified in the future.</p> <p> </p>