Scientist Contractor- Translational Biology

<p><strong><span data-contrast="auto">Position Summary:</span></strong><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></p> <p><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span><span data-contrast="auto">We are seeking a highly skilled and motivated Contract Scientist – Translational Biology to support mechanistic hypothesis testing and sample generation for rilparencel, our autologous kidney cell therapy currently being evaluated in a Phase 3 clinical study, as well as next-generation cell therapy programs. This role will focus on primary human tissue/cell culture models (BSL-2) and the execution of multi-condition studies that generate high-quality samples for downstream molecular and NGS-based readouts. In partnership with the Translational Biology team, this scientist will contribute to experimental design and method development for workflows involving small human biopsy-derived samples, with an emphasis on reproducible execution, rigorous sample tracking, and clear communication of results. </span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></p> <p><strong><span data-contrast="auto">Essential Duties & Responsibilities:</span></strong><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></p> <p><strong><span data-contrast="auto">Primary Human Tissue Culture (BSL-2) & Model Execution</span></strong><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></p> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="1" data-aria-level="2"><span data-contrast="auto">Establish, maintain, and troubleshoot primary human kidney tissue/cell cultures using strict aseptic technique and contamination prevention.</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <p><strong><span data-contrast="auto">Experimental Design & Method Development </span></strong><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></p> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="1" data-aria-level="2"><span data-contrast="auto">Develop and execute experimental plans with the translational team to test mechanistic hypotheses in vitro (e.g., media perturbations, multi-factor time courses), including appropriate controls and replicates.</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="2" data-aria-level="2"><span data-contrast="auto">Contribute to method development/optimization for primary tissue workflows (e.g., tissue handling, digestion/single-cell prep where applicable, culture conditions, sampling strategy, preservation), documenting protocol iterations and outcomes.</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="3" data-aria-level="2"><span data-contrast="auto">Execute complex study layouts with many conditions, timepoints, replicates, etc when required.</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <p><strong><span data-contrast="auto">Sample Processing & Molecular Assays</span></strong><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></p> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="1" data-aria-level="2"><span data-contrast="auto">Harvest tissue/cells and perform RNA/DNA/protein/metabolite extraction, quantification, and basic QC (e.g., yield, purity; integrity checks where applicable).</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="2" data-aria-level="2"><span data-contrast="auto">Run and troubleshoot core molecular assays, such as RT-qPCR, ELISA, Western blot, and/or reporter assays (e.g., luciferase), including control selection.</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="3" data-aria-level="2"><span data-contrast="auto">Prepare assay- and omics-ready sample sets with complete metadata for internal use and/or external partners, as needed.</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <p><strong><span data-contrast="auto">NGS Library Preparation & Omics Support</span></strong><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></p> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="1" data-aria-level="2"><span data-contrast="auto">Perform NGS library preparation for approaches such as RNA-seq, ATAC-seq, and CITE-seq (bulk and/or 10x single-cell), including essential QC steps (quantification, sizing where applicable, yield assessment).</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="2" data-aria-level="2"><span data-contrast="auto">Support handoff of libraries/samples to sequencing vendor pipelines, maintaining traceability of sample IDs, library indices, and processing conditions.</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <p><strong><span data-contrast="auto">Documentation & Communication</span></strong><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></p> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="1" data-aria-level="2"><span data-contrast="auto">Maintain high-quality experiment documentation (ELN) and accurate sample inventories (LIMS).</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="2" data-aria-level="2"><span data-contrast="auto">Participate in interpretation translational data to uncover key insights for cell therapy mechanism of action elucidation.</span><span data-ccp-props="{"201341983":0,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="3" data-aria-level="2"><span data-contrast="auto">Communicate findings effectively across cross-functional teams, including research, process & analytical development, and clinical translation groups.</span><span data-ccp-props="{"201341983":0,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="31" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="4" data-aria-level="2"><span data-contrast="auto">Contribute to regulatory filings, scientific publications, and presentations at key industry and academic conferences.</span><span data-ccp-props="{"201341983":0,"335559739":0,"335559740":288}"> </span></li> </ul> <p><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span><strong><span data-contrast="auto">Qualifications:</span></strong><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></p> <p><strong><span data-contrast="auto">Education/Training</span></strong><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></p> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="1" data-aria-level="2"><span data-contrast="auto">M.S. or Ph.D. in Cell Biology, Molecular Biology, Immunology, Bioengineering, or related field. </span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <p><strong><span data-contrast="auto">Required Experience & Skills</span></strong><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></p> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="1" data-aria-level="2"><span data-contrast="auto">Demonstrated hands-on experience with primary human tissue/cell culture and comfort working with human-derived materials in a BSL-2 environment.</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="2" data-aria-level="2"><span data-contrast="auto">Experience executing multi-condition in vitro experiments with strong attention to detail and sample tracking.</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="3" data-aria-level="2"><span data-contrast="auto">Experience with RNA/DNA/protein extraction and basic QC/quantification.</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="4" data-aria-level="2"><span data-contrast="auto">Experience with at least two of: qPCR/RT-qPCR, ELISA, Western blot, reporter assays.</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="5" data-aria-level="2"><span data-contrast="auto">Hands-on experience with NGS library preparation for one or more of: RNA-seq, ATAC-seq, CITE-seq (bulk and/or single-cell).</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="6" data-aria-level="2"><span data-contrast="auto">Able to work effectively in a fast-moving R&D environment with multiple parallel experiments and time-sensitive sample processing needs.</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <p><strong><span data-contrast="auto">Preferred</span></strong><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></p> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="1" data-aria-level="2"><span data-contrast="auto">Flow cytometry and/or FACS experience (sample prep, staining, acquisition and/or sorting workflows).</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="2" data-aria-level="2"><span data-contrast="auto">Familiarity with common bioinformatics/QC concepts and tools used in sequencing workflows; familiarity with at least one scripting language (Python and/or R).</span><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559739":0,"335559740":288}"> </span></li> </ul> <p><strong><span data-contrast="auto">Skills:</span></strong><span data-contrast="auto"> </span><span data-ccp-props="{"201341983":0,"335559739":0,"335559740":288}"> </span></p> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="1" data-aria-level="2"><span data-contrast="auto">Ability to work in a fast-paced environment and collaborate across disciplines</span><span data-ccp-props="{}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="2" data-aria-level="2"><span data-contrast="auto">Strong team player</span><span data-ccp-props="{}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="3" data-aria-level="2"><span data-contrast="auto">Goal-oriented mindset</span><span data-ccp-props="{}"> </span></li> </ul> <ul> <li data-leveltext="o" data-font="Courier New" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":1440,"335559991":360,"469769226":"Courier New","469769242":[9675],"469777803":"left","469777804":"o","469777815":"hybridMultilevel"}" data-aria-posinset="4" data-aria-level="2"><span data-contrast="auto">Ability to focus efforts & complete deliverables on-time</span><span data-ccp-props="{}"> </span></li> </ul> <ul> <li data-leveltext="" data-font="Symbol" data-listid="32" data-list-defn-props="{"335552541":1,"335559685":720,"335559991":360,"469769226":"Symbol","469769242":[8226],"469777803":"left","469777804":"","469777815":"hybridMultilevel"}" data-aria-posinset="5" data-aria-level="1"><strong><span data-contrast="auto">Certifications/Licenses:</span></strong><span data-contrast="auto"> Licenses / Certification welcome</span><span data-ccp-props="{}"> </span></li> </ul> <p><span data-ccp-props="{"201341983":0,"335557856":16777215,"335559685":720,"335559739":0,"335559740":288}"> </span></p><div class="content-conclusion"><p>ProKidney is an equal employment opportunity employer and does not discriminate against any applicant because of race, creed, color, age, national origin, ancestry, religion, gender, sexual orientation, disability, genetic information, veteran status, military status, application for military service or any other class protected by state or federal law.</p> <p>Applicants must be eligible to work in the United States without the need for work visa or residency sponsorship.</p></div>