Scientist

Contribute to experimental planning, implementation, and data analysis; Independently design plasmid maps, primers and cloning strategies for antibodies and fusion proteins; Use ExpiCHO transient transfection and expression system to produce new proteins; Purify protein from ExpiCHO expression system and use analytical instrument including HPLC, AKTA to characterize protein quantity, quality and purity; Design mutations to change certain properties of target protein, including receptor binding, stability, and productivity; Use Octet Red96 instrument to perform binding assays and measure the dissociation constant; Perform cell-based binding and functional screening assays to ID target specific antibody binders; Generate stable expression cell lines for cell based assays using pAS-puro vector and puromycin selection; Support antibody discovery team and perform phage display library construction and screening; Design and perform cell-based assays, including cell surface staining, signaling assay and flow cytometry; Prepare reports and present in weekly group meeting.