Molecular Biology/Plasmid Constructs Co-Op

Legend Biotech is seeking a Molecular Biology/Plasmid Constructs Co-Op as part of the R&D team based in Philadelphia, PA.

 

Role Overview

 

The Molecular Biology/Plasmid Constructs Co-Op will contribute to the design and functional validation of synthetic and natural promoters for T-cell gene expression, leveraging bioinformatics tools and molecular biology techniques. This role combines computational analysis with hands-on lab work to advance gene regulation strategies in cutting-edge gene therapy research.

Co-Op Duration: 6-month (Summer to Fall) with possible extension.

 

Key Responsibilities

• Assist in designing synthetic and natural promoters for T-cell specific gene expression using bioinformatics tools and literature mining.
• Perform cloning of promoter sequences into lentiviral vectors using standard molecular biology techniques (PCR, restriction digestion, ligation, Gibson assembly).
• Maintain mammalian cell lines and primary T cells; support transfection and transduction experiments.
• Conduct reporter assays (e.g. luciferase, GFP) to evaluate promoter strength and specificity in T cells or cell lines.
• Analyze experimental data using software such as GraphPad Prism, Excel, and basic statistical methods.
• Maintain accurate lab notebooks and present findings in team meetings. 

 

• Currently enrolled in Graduate programs (Master’s) in bioinformatics, data science, computer science, computational biology, or related field
• Basic understanding of molecular biology and cell culture principles
• Strong interest in promoter engineering and gene regulation with an understanding of transcriptional control mechanisms
• Experience with bioinformatics tools or programming languages (Python, R) is a plus
• Familiarity with laboratory techniques such as pipetting, buffer preparation, and aseptic handling.
• Strong attention to detail, organizational skills, and ability to work independently and in a team environment
• Excellent communication and documentation skills. 

 

What we offer:

• Exposure to cutting-edge gene therapy and synthetic biology.
• Practical experience in promoter engineering and functional validation.
• Mentorship from experienced scientists in a collaborative team setting. 

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